Purpose: The study aims to evaluate the potential anticancer properties of paeonol, a bioactive compound extracted from Cynanchum paniculatum (Bge.) Kitag. Methods: In this study, we investigated its cytotoxic and anti-migratory effects on four human breast cancer cell lines with different receptor profiles: MCF-7, ZR-75-1, SK-Br-3, and MDA-MB-231 (triple-negative). Investigation of signaling pathways associated with cell migration or cytotoxicity through Western blot analysis. Results: LC_(50) values ranged from 1.2 to 1.8 mM. Time-dependent cytotoxicity analysis showed sustained growth suppression in MCF-7, ZR-75-1, and SK-Br-3 cells, while MDA-MB-231 cells recovered after initial reduction. Wound healing assays demonstrated that paeonol significantly inhibited cell migration after 24 hours in all four cell lines; however, this effect diminished after 5 days. Western blot analysis revealed increased cleaved caspase-3 expression in MCF-7 cells, indicating apoptosis induction, whereas caspase-9 upregulation was significant only in SK-Br-3 cells. Notably, E-cadherin and MMP-2/9 expressions remained unchanged, suggesting migration suppression occurs through alternative pathways. Conclusions: These results suggest that paeonol exerts antitumor effects through receptor-independent apoptotic mechanisms and migration inhibition, with therapeutic potential especially against triple-negative breast cancer. Further mechanistic studies and in vivo validation are warranted to support paeonol as a candidate for breast cancer therapy.
【Keywords】
Paeonol ； breast cancer ； apoptosis ； migration ； triple-negative breast cancer
291-2 探討中藥徐長卿之有效成分丹皮酚對於 乳癌細胞株之影響
