中醫藥研究論叢

212.1 牛膝改善大鼠學習記憶障礙之研究
TJ TCM.21(2) : 01-08, 2018J TCM.21(1) : 01-14, 2018
牛膝改善大鼠學習記憶障礙之研究
The Study of Achyranthes Bidentata on Learning and Memory Impairment in Rats
吳龍源1* 呂峻安1 吳啟瑞2 蔡汎修1 蔡金川1,3 林立偉1*
1 義守大學學士後中醫學系,高雄,台灣
2 中國醫藥大學中國藥學暨中藥資源學系,台中,台灣
3 財團法人義大醫院中醫部,高雄,台灣
【摘要】
背景:阿茲海默症主要臨床症狀為記憶能力喪失,其病理特徵為老年斑,而老年斑主因類澱粉樣蛋白(amyloid β-peptide) 沉積所致,基於此理論本實驗設計應用amyloid β peptide (Aβ1-40) 來誘發阿茲海默症學習障礙之動物模型,並以水迷宮來評估中藥牛膝改善學習記憶障礙的作用。
材料& 方法:本計畫以80% 乙醇及水進行藥材萃取及分離,分別提取純化而製得懷牛膝皂苷提取物(ABS) 及懷牛膝多醣體提取物(ABP);後進行對Aβ1-40 誘導大鼠記憶障礙改善作用之評估。
結果:Aβ1-40 可造成大鼠於水迷宮之操作障礙暨短期記憶與參考記憶之障礙。ABS 50 mg/kg 可改善Aβ1-40 側腦室輸注所導致水迷宮空間操作暨參考記憶能力之障礙;而ABP 100 mg/kg 也能改善Aβ1-40 側腦室輸注所導致水迷宮空間操作暨參考記憶能力之障礙。
結論:懷牛膝之成分ABS ABP 能改善Aβ1-40 誘導大鼠水迷宮學習記憶障礙,可供中醫藥臨床基礎研究參考。
【關鍵詞】類澱粉樣蛋白、阿茲海默症、水迷宮、牛膝
Lung-Yuan WU1* Chun-An Lu1 Chi-Rei Wu2 Fan-Shiu Tsai1
Chin-Chuan Tsai1,3 Li-wei Lin1*
1The school of Chinese Medicine for post baccalaureate, I-Shou University, Kaohsiung, Taiwan
2Department of Chinese medicine of E-DA Hospital, Kaohsiung, Taiwan
3Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College
of Pharmacy, China Medical University, Taichung, Taiwan
【Summary】
PurposeAlzheimer’s disease (AD) is characterized by neuropathological hallmarks such as senileplaque and neurofibrillary tangles caused by the amyloid β peptide (Aβ1-40). For this reason, we demonstratedthe role of the improve effect of Achyranthesbidentata against continuous intracisternal injection of Aβ1-40 in rats
Materials & MethodsThis present result obtained total saponins and total polysaccharides extracted from Actyranthes bidentate (ABS and ABP) by 80% ethanol and distilled water. We further investigated the effects of ABS and ABP on the memory function caused by Aβ1-40 in rats. Intracisternal injection of Aβ1-40
caused the dysfunction of memory function on water maze.
ResultsPretreatment with ABS at a dosage of 50 mg/kg or ABP at 100 mg/kg reversed the dysfunction of memory function on water maze caused by intracisternal injection of Aβ1-40.
ConclusionsABS and ABP can improve the learning and memory impairment of rat water maze induced by Aβ1-40, which can be used as a reference for clinical basic research of traditional Chinese medicine.
Keywords amyloid β peptide, Alzheimer’s disease, water maze, Actyranthes bidentate